| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1351 | Leptospira biflexa | AAAATGGCCTGCAACGGG | CCAGGCAATCGACCACAGA | 58.94 | 59.70 | 222 | 82 |
100.00%
|
100.00%
|
| Primer 1352 | Leptospira biflexa | AAAATGGCCTGCAACGGG | CACCAGGCAATCGACCACA | 58.94 | 60.30 | 224 | 82 |
100.00%
|
100.00%
|
| Primer 1353 | Leptospira biflexa | TGTGGTTGGTGTCCTGCA | TGGGCTGTGGTGGTTTGT | 59.00 | 59.31 | 190 | 82 |
100.00%
|
100.00%
|
| Primer 1354 | Leptospira biflexa | TCCGTTCGACAACCACCTG | CCCCTTCCCAACGAAAGCA | 59.93 | 60.23 | 169 | 82 |
100.00%
|
100.00%
|
| Primer 1355 | Leptospira biflexa | GCGTTTTGAGTCTGTGGGC | CCCCTTCCCAACGAAAGCA | 59.72 | 60.23 | 243 | 82 |
100.00%
|
100.00%
|
| Primer 1356 | Leptospira biflexa | ATCCCGTTGGCGTATGCA | TGTTCCCGACTGGCATTGG | 59.41 | 60.30 | 272 | 82 |
100.00%
|
100.00%
|
| Primer 1357 | Leptospira biflexa | TGGCCAAGCAGATCGACTC | TTGGCAGCGATTCCAAGC | 59.78 | 58.72 | 261 | 82 |
100.00%
|
100.00%
|
| Primer 1358 | Leptospira biflexa | TGGCCAAGCAGATCGACTC | TTTGGCAGCGATTCCAAGC | 59.78 | 59.41 | 262 | 82 |
100.00%
|
100.00%
|
| Primer 1359 | Leptospira biflexa | CGTTGCCATCGGAGCTTTC | TGCTTCGTATCCTTCCGCA | 59.57 | 59.11 | 157 | 82 |
100.00%
|
100.00%
|
| Primer 1360 | Leptospira biflexa | ACATCGGTCAGCTGTGCA | AGAATGGCCAGAGTGGTCC | 59.26 | 59.01 | 227 | 82 |
100.00%
|
100.00%
|